The red led in the magpix or laser in the luminex 100200 is responsible for classifying the beads. Bioagilytix employs biorads luminexbased bioplex multiplex system with laser based technology and luminex flexmap 3d systems to run beadbased immunoassays. The second laser determines the magnitude of the pederived signal, which is in direct proportion to the amount of analyte bound. The second is a flow cytometrybased instrument, the luminex 100200 analyzer, which integrates key xmap detection components, such as lasers, optics, fluidics, and highspeed digital signal processors. Flexible, intuitive, and affordable cytometry for all your needs watch an overview video on the guava easycyte systems our microcapillary flow cytometry systems are simpler to operate than traditional sheathfluid based instruments and are far easier to maintain. Introduction to xmap technology and milliplex assay panels. Intended use perform multiple assays with a single sample with the athena multilyte test analyzer, built by luminex corporation on the luminex 100200 system using xmap technology. The procedures are presented as stepbystep instructions and assume basic knowledge of xponent software and xmap technology. Flexmap 3d multiplexing instrument from luminex corporation. The muse cell analyzer packs 3parameter analysis into a compact, easy to use benchtop device, making flow cytometry accessible to anyone, anytime. Luminex assays supportgetting started thermo fisher. Clinical diagnostics instruments luminex 100200 system. The software s graphical user interface follows the typical assay workflow but can be customized to meet the needs of a specific laboratory.
See the xponent license agreement for more information. This multiplex, fluorescent, beadbased system offers a simple and efficient alternative to ifa and elisa testing. The bioplex 3d suspension array system is the next generation multiplexing platform based on xmap technology. The softwares graphical user interface follows the typical assay workflow, but can be customized to meet the needs of a specific laboratory. Additionally, while the luminex 100200 relies on the principles.
Luminex xmap technology combines advanced fluidics, optics, and digital signal. The luminex 100200 analyzer integrates key xmap detection components such as lasers, optics, fluidics and highspeed digital signal processors. Do you know where the serial number and firmware version are located in luminex xponent software. The first laser is a red diode laser that classifies beads region based on color and hence identifies which protein is being measured. The system accommodates 96 and 384well plate formats for. The luminex systems are a series comprised of three instrument modelsmagpix, lx200 and flexmap 3d. See finding the current xponent license key below for details.
Designed for easeofuse, xponent enables both new and advanced users to set up and run assays in a minimal amount of time. Luminex lx200 with xponent software zeus scientific. Luminex receives barda contract to support development of. The luminex 100200 system employs flow cytometry in the measurement of the signal. Flexmap 3d system advanced and versatile multiplexing platform. These platforms give us the ability to multiplex many different assays simultaneously, providing greater information from less sample volume and in a shorter timeframe than required. Luminex instrumentation overview instruments magpix luminex 100200 flexmap 3d software xponent xponent xponent optics ledccd camera lasersapdspmts lasersapdspmts hardware fluorescent imager flow cytometry based flow cytometry based bead compatibility magplex magplextag magplex microplex magplextag seromap lumavidin magplex microplex.
Austin, texas, march, 2020 prnewswire luminex corporation nasdaq. The software should be fully licensed or uninstalled before the trial period expires. Turn on the luminex 200 analyzer, xy platform, and, if used, the luminex sheath delivery system. The luminex 200 and flexmap 3d reader combines two lasers, fluidics, and. Please contact luminex technical support to obtain a permanent license. The elx50 microplate strip washer biotek instruments used in these experiments was configured with a 96well flat magnet pn 7103016. Fast read time, compatibility with 96 and 384well plates, and ease of integration with frontend automation systems make the flexmap 3d the. Use our product documents resource to find product manuals, help guides, coq, msds and more. State of the art optics, electronics, and software make the labscan systems capable of rapidly processing up to 96 samples.
In order for luminex technical support to generate a permanent license key for the luminex xponent software or enable a purchased xponent module lis, automation, security, 21 cfr, we need the following pieces of information. The third component is the xponent software, which is designed. This system contains electrical, mechanical, and laser components that, if handled. We are excited to be at amp 2018 in san antonio, tx. The systems xponent software is designed for protocolbased data. Austin, texas, may 8, 2020 prnewswire luminex corporation nasdaq. Jan 15, 2018 heres how to start up the luminex 200 instrument 1. The luminex 100200 system is the combination of three core xmap technologies. This flexible software package controls the, luminex 200. Luminex analyzer, the classification laser red, 635 nm excites the internal. Luminex xmap technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. Bioplex 3d suspension array system life science research. The luminex magpix instrument system is the simplest, most affordable, and most compact luminex instrument.
Automated washing of multiplex bead based assays for the. Luminex systems installation qualification iq and operational qualification oq iqoq protocol protocol no. User customizationthe default graphical user interface follows a typical assay workflow, and it can be customized to meet the needs of individual. Luminex technology employs microsphere beads, either polystyrene or paramagnetic, that are colorcoded. Expanded multiplexing capability, faster time to results, and automation capability make it the platform of choice for highthroughput testing for nucleic acid and protein applications. Heres how to start up the luminex 200 instrument 1.
The third component is the xponent software, which is designed for protocolbased. The first is xmap microspheres, a family of fluorescently dyed micronsized polystyrene microspheres that act as both the identifier and the solid surface to build the assay. There are currently two flow cytometrybased instruments available from luminex or through its partners, the luminex 200 and the flexmap 3d. Luminet monitor is a free software pack that will increase the comfort of working with luminex equipment and together with the easytouse web interface for ethernetdmx converters, it adds high value to your project monitoring and preparation.
The luminex 100200 system is a flexible analyzer based on the principles of flow cytometry. Feature introduction to luminex xmap technology and. The green led in the magpix or laser in the luminex 100200 with the filters produce the reporter fluorescence which identifies the analytes captured in the assay. This system is a flexible analyzer based on the principles of flow cytometry which integrates key xmap detection components such as lasers, optics, advanced. Luminex corporation luminex regarding the use of the xponent software product. Laser safety online training this 15 minute course shows the user information on laser safety in a selfpaced and interactive online setting where the end.
The second is a flow cytometrybased instrument, the luminex 100200 analyzer, which. Luminex provides update on sarscov2 validation testing. Each protein detected requires a different bead region to differentiate between analytes. While maudio will continue to field questions about torq software, we will no longer promote or sell torq software globally. Luminex 200 system with xponent software from thermo fisher. Feb 03, 2017 luminex xponent software provides data acquisition and analysis for luminex xmap instruments. We encourage torq customers, as well as all customers interested in dj products, to visit maudio. System startup verify that the following procedures were performed when the magpix system was turned on for the first time. The system enables your laboratory to simultaneously measure up to 100 analytes in a single well. The labscan3d and labscan 100 systems are based on luminex xmap technology and use luminex xponent software for data acquisition. Beads are read on a dual laser flowbased detection instrument, such as the luminex 200 or flexmap analyzer. Luminex 200 multiplexing instrument from luminex corporation description the luminex 200 detects up to 100 protein or nucleic acid analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents. Additionally, please refer to our luminex assays support center for data analysis tips and tricks. The xponent application is licensed in trial mode for 30 days.
The ability to use either magnetic or polystyrene beads makes this instrument a popular choice for laboratories needing greater flexibility. Enduser license agreement eula for luminex xponent software this luminex enduser license agreement eula is a legal agreement between you either an individual or a single entity, also referred herein as you the enduser and luminex corporation luminex regarding the use of the xponent software product provided to. All luminex instruments come with xponent data analysis software that can be used. Syringe cylinder with seal cn001 syringe seal qty 4 cn001401 cable, usb cn001801 waste bottle cn001201 luminex sd luminex sheath cns00101 delivery system software customer product description number cnsw0801 luminex 100 is version 2. Software updates are available for your luminex magpix system. The luminex instrument is outfitted with two lasers. In these systems, beads are interrogated individually. Flexmap 3d the flexmap 3d is luminex s most advanced and versatile multiplexing platform. This imagebased multiplexing unit performs up to 50 different tests in a single reaction volume and reads a 96wellplate in just 60 minutes. Designed for easeofuse, the software allows the novice or occasional user and advanced user alike, to set up and run an assay in a minimal amount of time.
The third component is the xponent software, which is designed for protocolbased data acquisition with robust data regression analysis. With highly sensitive and configurable optics, researchers benefit from. All luminex instrumentation sold by biotechne arrives preinstalled with xponent data acquisition and analysis software. Alternatively, kits can be used with the luminex 100200, luminex flexmap 3d, or biorad bioplex, dual laser, flowbased sorting and detection platforms.
Flexmap 3d multiplexing instrument from luminex corporation description the flexmap 3d detects up to 500 protein or nucleic acid analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents. Magnetic luminex performance assay multiplex kits are designed for use with the luminex magpix ccd imager. Flexmap 3d the flexmap 3d is luminexs most advanced and versatile multiplexing platform. Fast read time, compatibility with both 96 and 384well plates, and ease of integration with frontend automation systems make the flexmap 3d the platform of choice for high throughput applications. Luminex 100200 for life science research standard for. This information is presented to support researchers who use magpix, 100200, and flexmap 3d luminex systems. Modular and flexibledesigned for ease of use, xponent software allows novice, occasional, and advanced users set up and run assays in minimal amounts of time. Milliplex analyst software milliplex analyst is a powerful multiplex data analysis software package developed by millipore in conjunction with vigenetech to integrate with the luminex xponent multiplexing software xponent will continue to be used for data acquisition pdf and excel export compatible automatic calculation of minmaxdc. They utilize small sample volumes, generate minimal waste, and have lower operating costs. Bring your biomarkers to life with the established leaders in.
The software s graphical user interface follows the typical assay workflow, but can be customized to meet the needs of a specific laboratory. If the trial period expires, the application will become inoperable. One laser classifies the bead and determines the analyte that is being detected. A userfriendly touchscreen interface, intuitive cell analysis software, and optimized assays work to simplify your research. Designed for easeofuse, the software allows either a novice or advanced user to set up and run an assay in a minimal amount of time. Austin, texas, march 31, 2020 prnewswire luminex corporation nasdaq. This protocol is a stepbystep method for setting up the xponent 3. Luminex corporation 12212 technology boulevard austin, texas 787276115 u. The systems xponent software is designed for protocolbased data acquisition with robust data regression analysis. This manual describes the features and functions of xponent for magpix. The luminex 100200 and flexmap 3d are class 1i laser products. Luminex 100200 sets the standard for multiplexing, with over 11,000 units sold globally and more than fifty 510kcleared assays.
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